Job Description Summary
We are seeking a candidate who will assist our ongoing research directed toward understanding mechanisms of fibrosis. The candidate will perform supervised work on several projects using mice, human samples, and cultured cells. General duties will include breeding, weaning and genotyping genetically altered mice, assisting in collection, processing and cataloging tissue samples from mice, culture of primary cells, RNA and protein extraction, performing various biologic analyses (gel electrophoresis, Western blotting, ELISA, immunohistochemistry and immunocytochemistry, qRT-PCR, etc.), analyzing protein levels in blood of patients with pulmonary fibrosis and controls, and participating in laboratory discussions, organization and regulatory procedures. Maintenance of up-to-date, comprehensive and neat laboratory records will be required. Will require occasional weekend work.
Under supervision from the lab manager and principal investigator, candidate will perform complex biological procedures and analyses in a basic science laboratory. All of the experiments require meticulous technique and the ability to optimize and troubleshoot a variety of different protocols. The candidate will be expected to help prepare experimental results for presentation and publication.Entity
Medical University of South Carolina (MUSC - Univ)Worker Type
EmployeeWorker Sub-Type?
Research GrantCost Center
CC000975 COM DOM Rheumatology CCPay Rate Type
HourlyPay Grade
University-04
Pay Range
$31,561 - $44,977 - $58,393Scheduled Weekly Hours
40Work Shift
Job Description
Job Duties:
35% - Analyze blood samples for target proteins using ELISA
- Catalog samples from controls and patients
- Aliquot samples to avoid freeze-thaw cycles
- Measure levels of several proteins using ELISA
25% - Perform variety of cell and molecular biological techniques, record data. Analyze experimental results using appropriate software; help prepare experimental results for presentation and publication.
10% - Maintain mouse colony and perform experimental and routine procedures using established approvals and protocols.
10% - Develop and test new laboratory methods to meet changing needs; troubleshoot experimental protocols.
10% - Assist in culture of primary cells and cell lines.
10% - Maintain laboratory supplies; prepare media, buffers, and other reagents as needed. Keep complete laboratory records/notebook.
Additional Knowledge, Skills, and Abilities Preferred:
- Knowledge about small-volume fluid handling, RNA procedures, tissue culture/sterile technique, mouse manipulation, ELISA, and general cell, molecular, biochemical, and/or immunological methods is preferred, and experience in these areas is desired.
- The candidate should possess strong organizational and communication skills, a talent for managing diverse tasks and more than one project, and an ability to work well in a team.
- Candidate should be highly motivated, detail oriented, and committed to excellence. The ideal candidate will have a keen intellectual interest in the science behind the projects and a demonstrated commitment to increasing their background knowledge about their work. An equivalent combination of education and experience may substitute for stated requirements.
- Experience with molecular biology techniques and primary sterile cell culture techniques preferred. Proficiency with computers and experience with spreadsheet, statistical, and graphical software are also preferred. Previous experience handling mice is a plus.
Additional Job Description
Minimum Requirements: A high school diploma and two years work experience in a clinical or research setting; a bachelor's degree in chemistry, biology, or other natural, life or health care science may be substituted for the two years of required experience.
Physical Requirements: (Note: The following descriptions are applicable to this section: Continuous - 6-8 hours per shift; Frequent - 2-6 hours per shift; Infrequent - 0-2 hours per shift) Ability to perform job functions in an upright position. (Frequent) Ability to perform job functions in a seated position. (Continuous) Ability to perform job functions while walking/mobile. (Frequent) Ability to climb, using step stool. (Infrequent) Ability to work in temperature extremes (32° F to 100° F) when required due to assignments in cold rooms and/or foreign countries. (Infrequent) Ability to work in confined/cramped spaces. (Continuous) Ability to bend at the waist. (Frequent) Ability to twist at the waist. (Frequent) Ability to fully use both hands/arms. (Continuous) Ability to perform repetitive motions with hands/wrists/elbows and shoulders. (Frequent) Ability to fully use both legs. (Frequent) Ability to reach in all directions, stretch, bend, while moving items/materials from both sitting and standing positions. (Frequent) Ability to maintain good olfactory sensory function/distinction. (Continuous) Ability to lift materials up to 20 lbs., unassisted. (Infrequent) Ability to lift and carry 4 liter waste material bottles a distance of 30 feet, unassisted. (Frequent) Ability to lift and carry 25-30 lbs. jugs of liquid a distance of 100 feet, unassisted. (Infrequent) Ability to lift file boxes, from floor level to counter tops, unassisted. (Frequent) Ability to push/ Ability to lift 40 lbs. centrifuge rotors from floor level to height of 40 inches, then down 20 inches into centrifuge drum, unassisted. (Infrequent) Ability to lift 25 lbs. boxes, from floor level to maximum height of 72 inches, unassisted. (Infrequent) Ability to push/pull file boxes from one location to another at floor level. (Frequent) Ability to maneuver heavy equipment on bench tops, 50 lbs. or greater, unassisted. (Frequent) Ability to perform gross motor functions with frequent fine motor movements. (Continuous) Ability to maintain 20/40 vision, corrected, in one eye or with both eyes. (Continuous) Ability to match or discriminate between colors, normally. (Continuous) Ability to hear and recognize various alarms emitting from work area systems. (Continuous) Ability to hear and/or understand conversations. (Continuous) Ability to work in a darkroom for processing film, etc. (Continuous) Ability to be qualified physically for respirator use, initially and annually. (Continuous)
Additional New Requirements: Ability to work rotating shifts as required. Ability to work overtime as required. Ability to learn and use new processes, tools and equipment as required.
If you like working with energetic enthusiastic individuals, you will enjoy your career with us!
The Medical University of South Carolina is an Equal Opportunity Employer. MUSC does not discriminate on the basis of race, color, religion or belief, age, sex, national origin, gender identity, sexual orientation, disability, protected veteran status, family or parental status, or any other status protected by state laws and/or federal regulations. All qualified applicants are encouraged to apply and will receive consideration for employment based upon applicable qualifications, merit and business need.
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